Which of these factors does not govern the stability of a conformation in acyclic compounds?

The increasing order of nucleophilicity of the following nucleophiles is :
(a) CH₃CO₂^$$-$$           (b) H₂O
(c) CH₃SO₃^$$-$$           (d) $$\mathop O\limits^ - H$$

The principle of column chromatography is :

The increasing order of the reactivity of the following compounds towards electrophilic aromatic substitution reactions is :