A linear octasaccharide (molar mass $=1024 \mathrm{~g} \mathrm{~mol}^{-1}$ ) on complete hydrolysis produces three monosaccharides: ribose, 2-deoxyribose and glucose. The amount of 2-deoxyribose formed is $58.26 \%(\mathrm{w} / \mathrm{w})$ of the total amount of the monosaccharides produced in the hydrolyzed products. The number of ribose unit(s) present in one molecule of octasaccharide is $\qquad$ .
Use: Molar mass $\left(\right.$ in g $\left.\mathrm{mol}^{-1}\right)$ : ribose $=150,2$-deoxyribose $=134$, glucose $=180$;
Atomic mass (in amu): $\mathrm{H}=1, \mathrm{O}=16$
For a double strand DNA, one strand is given below:

The amount of energy required to split the double strand DNA into two single strands is _______ kcal $\operatorname{mol}^{-1}$.
[Given: Average energy per H-bond for A-T base pair $=1.0 ~\mathrm{kcal}~ \mathrm{mol}^{-1}$, G-C base pair $=1.5 ~\mathrm{kcal}$ $\mathrm{mol}^{-1}$, and A-U base pair $=1.25 ~\mathrm{kcal} ~\mathrm{mol}^{-1}$. Ignore electrostatic repulsion between the phosphate groups.]

If the absolute values of the net charge of the peptide at $\mathrm{pH}$ $=2$, $\mathrm{pH}=6$, and $\mathrm{pH}=11$ are $\left|Z_1\right|,\left|Z_2\right|$, and $\left|Z_3\right|$, respectively, then what is $\left|Z_1\right|+\left|Z_2\right|+\left|Z_3\right|$?
