1
NEET 2021
MCQ (Single Correct Answer)
+4
-1
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
A
Annealing, Denaturation, Extension
B
Denaturation, Annealing, Extension
C
Denaturation, Extension, Annealing
D
Extension, Denaturation, Annealing
2
NEET 2021
MCQ (Single Correct Answer)
+4
-1
Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for $$\beta$$-galactoside production and the recombinant plasmid is inserted in an E.coli strain
A
It will be able to produce a novel protein with dual ability
B
It will not be able to confer ampicillin resistance to the host cell
C
The transformed cells will have the ability to resist ampicillin as well as produce $$\beta$$-galactoside
D
It will lead to lysis of host cell
3
NEET 2021
MCQ (Single Correct Answer)
+4
-1
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
A
Ligation
B
Annealing
C
Extension
D
Denaturation
4
NEET 2021
MCQ (Single Correct Answer)
+4
-1
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :
A
Poly (A) tail sequences
B
Degenerate primer sequence
C
Okazaki sequences
D
Palindromic Nucleotide sequences
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