1
NEET 2021
MCQ (Single Correct Answer)
+4
-1
Change Language
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
A
Annealing, Denaturation, Extension
B
Denaturation, Annealing, Extension
C
Denaturation, Extension, Annealing
D
Extension, Denaturation, Annealing
2
NEET 2021
MCQ (Single Correct Answer)
+4
-1
Change Language
Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for $$\beta$$-galactoside production and the recombinant plasmid is inserted in an E.coli strain
A
It will be able to produce a novel protein with dual ability
B
It will not be able to confer ampicillin resistance to the host cell
C
The transformed cells will have the ability to resist ampicillin as well as produce $$\beta$$-galactoside
D
It will lead to lysis of host cell
3
NEET 2021
MCQ (Single Correct Answer)
+4
-1
Change Language
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
A
Ligation
B
Annealing
C
Extension
D
Denaturation
4
NEET 2021
MCQ (Single Correct Answer)
+4
-1
Change Language
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :
A
Poly (A) tail sequences
B
Degenerate primer sequence
C
Okazaki sequences
D
Palindromic Nucleotide sequences
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