Biotechnology: Principles and Processes · Biology · NEET

In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

Polymerase chain reaction (PCR) amplifies DNA following the equation.

Identify the part of a bio-reactor which is used as a foam braker from the given figure.

The blue and white selectable markers have been developed which differentiate recombinant colonies from nonrecombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.

Given below are two statements about this method:

Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies.

Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.

In the light of the above statements, choose the most appropriate answer from the options given below:

Silencing of specific mRNA is possible via RNAi because of

Which of the following enzyme(s) are NOT essential for gene cloning?

A. Restriction enzymes

B. DNA ligase

C. DNA mutase

D. DNA recombinase

E. DNA polymerase

Choose the correct answer from the options given below:

Given below are two statements:

Statement 1 : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below :

Match List-I with List - II :

Choose the correct answer from the options given below:

Cryopreservation technique is used for :

Identify the incorrect statement related to gel electrophoresis.

Given below are two statements :

Statement I: In the lac operon, the z gene codes for beta-galactosidase which is primarily responsible for the hydrolysis of lactose into galactose and glucose.

Statement II: In addition to lactose, glucose or galactose can also induce lac operon.

In the light of the above statements, choose the correct answer from the options given below :

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

The Bt toxin in genetically engineered Bt cotton kills the pest by:

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet$$^{\mathrm{R}}$$) gene in the pBR322 cloning vector.

Which of the following are correct about EcoRI?

A. Cut the DNA with blunt end

B. Cut the DNA with sticky end

C. Recognise a specific palindromic sequence

D. Cut the DNA between the base $$\mathrm{G}$$ and $$\mathrm{A}$$ when encounters the DNA sequence 'GAATTC'

E. Exonuclease

Choose the correct answer from the options given below:

Following are the steps involved in the process of PCR.

A. Annealing

B. Amplification (~1 billion times)

C. Denaturation

D. Treatment with Taq polymerase and deoxynucleotides

E. Extension

Choose the correct sequence of steps of PCR from the options given below :

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

The "Ti plasmid" of Agrobacterium tumefaciens stands for

Thermostable DNA polymerase used in PCR was isolated from :

Ligation of foreign DNA at which of the following site will result in loss of tetracyclin resistance of pBR322 :

Match List I with List II.

Choose the correct answer from the options given below :

Which of the following is not a cloning vector?

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A) : When a particular restriction enzyme cuts strand of DNA, overhanging stretches or sticky ends are formed.

Reason (R) : Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site.

In the light of the above statements, choose the correct answer from the options given below :

Separation of DNA fragments is done by a technique known as

The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :

Match List-I with List-II :

Choose the correct answer from the options given below:

Pathogenic bacteria gain resistance to antibiotics due to changes in their :

Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?

Refer to the following statements for agarose-gel electrophoresis:

(a) Agarose is a natural polymer obtained from sea-weed.

(b) The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA.

(c) The DNA migrates from negatively-charged electrode to the positively-charged electrode

(d) The DNA migrates from positively-charged electrode to the negatively-charged electrode.

Choose the most appropriate answer from the options given below :

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).

Assertion (A) : Polymerase chain reaction is used in DNA amplification.

Reason (R) : The ampicillin resistant gene is used as a selectable marker to check transformation.

In the light of the above statements, choose the correct answer from the options given below :

Which one of the following statement is not true regarding gel electrophoresis technique?

Given below are two statements:

Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.

Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.

In the light of the above statements, choose the most appropriate answer from the options given below :

Which of the following is not a desirable feature of a cloning vector?