Biotechnology: Principles and Processes · Biology · NEET

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Cryopreservation technique is used for :

Identify the incorrect statement related to gel electrophoresis.

Given below are two statements : Statement I: In the lac operon, the z gene codes for beta-galactosidase which is primarily responsible for the hydro...

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

The Bt toxin in genetically engineered Bt cotton kills the pest by:

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet$$^{\mathrm{R}}$$) gene in the pBR...

Which of the following are correct about EcoRI? A. Cut the DNA with blunt end B. Cut the DNA with sticky end C. Recognise a specific palindromic seque...

Following are the steps involved in the process of PCR. A. Annealing B. Amplification (~1 billion times) C. Denaturation D. Treatment with Taq polymer...

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

The "Ti plasmid" of Agrobacterium tumefaciens stands for

Thermostable DNA polymerase used in PCR was isolated from :

Ligation of foreign DNA at which of the following site will result in loss of tetracyclin resistance of pBR322 :

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Which of the following is not a cloning vector?

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : When a particular restrictio...

Separation of DNA fragments is done by a technique known as

The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct...

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Pathogenic bacteria gain resistance to antibiotics due to changes in their :

Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?

Refer to the following statements for agarose-gel electrophoresis: (a) Agarose is a natural polymer obtained from sea-weed. (b) The separation of DNA ...

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : Polymerase chain reaction i...

Which one of the following statement is not true regarding gel electrophoresis technique?

Given below are two statements: Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequenc...

Which of the following is not a desirable feature of a cloning vector?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

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Which of the following is not an application of PCR (Polymerase Chain Reaction)?

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for ...

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of ...

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :

In gel electrophoresis, separated DNA fragments can be visualized with the help of

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

Identify the wrong statement with regard to Restriction Enzymes

The specific palindromic sequence which is recognized by EcoRl is

The sequence that controls the copy number of the linked DNA in the vector, is termed

Match the organism with its use in biotechnology (a) Bacillus thuringiensis (i) Cloning vector (b) Thermus aquaticus (ii) ...

Choose the correct pair from the following

DNA precipitation out of a mixture of bimolecules can be achieved by treatment with :

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

The correct order of steps in Polymerase Chain Reaction (PCR) is

The process of separation and purification of expressed protein before marketing is called :

A gene whose expression helps to identify transformed cell is known as

DNA fragments are

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

The DNA fragments separated on an agarose gel can be visualised after staining with

Stirred-tank bioreactors have been designed for -

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Which of the following is not a component of downstream processing ?

Which of the following restriction enzymes produces blunt ends ?

Which of the following is not a feature of the plasmids ?

The taq polymerase enzyme is obtained from

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

Which of the following is a restriction endonuclease ?

The DNA molecule to which the gene of interest is integrated for cloning is called -

The cutting of DNA at specific locations became possible with the discovery of -

Which vector can clone only a small fragment of DNA ?

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

Commonly used vectors for human genome sequencing are :

During the process of isolation of DNA, chilled ethanol is added to :

The colonies of recombinat bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

Which of the following is not corretly matched for the organism and its cell wall degrading enzyme ?

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

Which one of the following represents a palindromic sequence in DNA ?

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it...

In genetic engineering, the antibiotics are used

Biolistics (gene-gun) is suitable for -

For transformation, micro - particles coated with DNA to be bombarded with gene are made up of

Which one is a true statement regarding DNA polymerase used in PCR ?

A single strand of nucleic acid tagged with a radioactive molecule is called

PCR and Restriction Fragment Length Polymorphism are the methods for

The figure below is the diagrammatic respesentation o the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain compo...

Which part would be most suitable for raising virus free plants for microporpagation ?

Which one of the following techniques made it possible to genetically engineer living organisms ?

There is a restriction endonuclease called EcoRl. What does "co" part in it stand for ?

Agarose extracted from sea weeds finds use in :

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it ? 5' ...............

In genetic engineering, a DNA segment (gene) of interest,is transferred to the host cell through a vector. Consider the following four agents (A-D) in...

Stirred-tank bioreactors have been designed for –

Which of the following are used in gene cloning ?

Restriction endonucleases are enzymes which –

Which one of the following is used as vector for cloning genes into higher organisms ?

DNA or RNA segment tagged with a radioactive moleculer is called –

Somaclones are obtained by : -

Which one of the following is commonly used in transfer of foreign DNA into crop plants ?

The linking of antibiotic resistance gene with the plasmid vector became possible with:

Gel electrophoresis is used for:

Two microbes found to be very useful in genetic engineering are-

Restriction endonuclease -

In transgenics expression of transgene in target tissue is determined by :-

Restriction endonucleases :-

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

In recent years, DNA sequences (nucleotide sequence) of mt-DNA and Y chromosomes were considered for the study of human evolution, because : -

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

Maximum number of bases in plasmids discovered so far : -

Which of the following cut the DNA from specific places ?

In lederberg's replica plating experiment what shall be used to obtain streptomycin resistant strain : -

A mutant strain of T4 – Bacteriophage, R-II, fails to lyse the E-Coli but when two strains RIIX and R-IIY are mixed then they lyse the E.Coli. What ma...

Plasmid has been used as vector because :

The bacteria generally used for genetic engineering is :