Biotechnology and It's Applications · Biology · NEET

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

Following are the steps involved in action of toxin in Bt. Cotton

A. The inactive toxin converted into active form due to alkaline pH of gut of insect.

B. Bacillus thuringiensis produce crystals with toxic insecticidal proteins.

C. The alkaline pH solubilises the crystals.

D. The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect.

E. The toxin proteins exist as inactive protoxins in bacteria.

Choose the correct sequence of steps from the options given below:

The capacity to generate a whole plant from any cell of the plant is called:

Given below are two statements:

Statement I : Bt toxins are insect group specific and coded by a gene cry IAc.

Statement II : Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect the inactive protoxin gets converted into active form due to acidic $$\mathrm{pH}$$ of the insect gut.

In the light of the above statements, choose the correct answer from the options given below:

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?

A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.

B. It may get integrated into the genome of the recipient.

C. It may multiply and be inherited along with the host DNA.

D. The alien piece of DNA is not an integral part of chromosome.

E. It shows ability to replicate.

Choose the correct answer from the options given below:

Which of the following are fused in somatic hybridization involving two varieties of plants?

Match List I with List II

Choose the correct answer form the options given below:

Which of the following statements is incorrect?

The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes :

Which of the following statement is incorrect about Agrobacterium tumifaciens?

Which of the following can act as molecular scissors?

Match List-I with List-II.

Choose the correct answer from the options given below :

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell

B. Cutting of DNA at specific location by restriction enzyme

C. Isolation of desired DNA fragment

D. Amplification of gene of interest using PCR

Choose the correct answer from the options given below :

Milk of transgenic ‘Cow Rosie’ was nutritionally more balanced product for human babies than natural cow milk because it contained:

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:

Statements related to human Insulin are given below.

Which statements is/are correct about genetically engineered Insulin?

(a) Pro-hormone insulin contain extra stretch of C-peptide

(b) A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them.

(c) Insulin used for treating Diabetes was extracted from Cattles and Pigs.

(d) Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone.

(e) Some patients develop allergic reactions to the foreign insulin.

Choose the most appropriate answer from the options given below :