Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet$$^{\mathrm{R}}$$) gene in the pBR322 cloning vector.

Which of the following are correct about EcoRI?

A. Cut the DNA with blunt end

B. Cut the DNA with sticky end

C. Recognise a specific palindromic sequence

D. Cut the DNA between the base $$\mathrm{G}$$ and $$\mathrm{A}$$ when encounters the DNA sequence 'GAATTC'

E. Exonuclease

Choose the correct answer from the options given below:

Following are the steps involved in the process of PCR.

A. Annealing

B. Amplification (~1 billion times)

C. Denaturation

D. Treatment with Taq polymerase and deoxynucleotides

E. Extension

Choose the correct sequence of steps of PCR from the options given below :

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of: