Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

Which of the following statements are not true regarding restriction endonucleases?

A. They are called molecular scissors.

B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.

C. They cut the DNA only at the centre of the palindromic sites.

D. They remove nucleotides only from the ends of DNA fragments.

E. They recognise specific palindromic base-pair sequences.

Choose the answer from the options given below :

Which of the following statements are correct with respect to DNA separation, isolation and visualization?

A. The cutting of DNA is done by molecular scissors.

B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.

C. The separated DNA fragments can be seen without staining when exposed to UV light.

D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light.

Choose the correct answer from the options given below :

Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards: