Arrange the following steps of DNA fingerprinting in a correct sequence.

A. Isolation of DNA and its digestion by restriction endonucleases.

B. Hybridisation using a labelled VNTR probe.

C. Transferring of separated DNA fragments to synthetic membranes.

D. Detection of hybridised DNA fragments by autoradiography.

E. Separation of DNA fragments by electrophoresis.

Choose the correct answer from the options given below :

Which of the following statements are correct with reference to packaging of DNA helix ?

A. Histones are organized to form a unit of eight molecules called histone octamer.

B. Histones are negatively charged basic proteins.

C. Histones are rich in the basic amino acid residues - lysine and arginine.

D. The positively charged DNA is wrapped around the histone octamer to form nucleosome.

E. The packaging of chromatin at higher levels requires an additional set of proteins called non-histone chromosomal proteins.

Choose the correct answer from the options given below :

The sixth mutant codon of beta globin gene causing polymerization of Haemoglobin and change in RBC shape is $\_\_\_\_$

Which chromosome in the human genome has the highest number of genes?