1
AIPMT 2012 Mains
MCQ (Single Correct Answer)
+4
-1
Change Language
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents ?
AIPMT 2012 Mains Biology - Biotechnology: Principles and Processes Question 70 English
A
C - Extension in the presence of heat stable DNA polymerase
B
B - Denaturation at a temperature of about 98ºC separating the two DNA strands
C
A - Denaturation at a temperature of about 50ºC
D
A - Annealing with two sets of primers
2
AIPMT 2012 Mains
MCQ (Single Correct Answer)
+4
-1
Change Language
In genetic engineering, the antibiotics are used
A
As sequences from where replication starts
B
As selectable markers
C
To keep the cultures free of infection
D
To select healthy vectors
3
AIPMT 2012 Mains
MCQ (Single Correct Answer)
+4
-1
Change Language
Which one of the following represents a palindromic sequence in DNA ?
A
5'-GAATTC-3'
3'-CTTAAG-5'
B
5'-GATACC-3'
3'-CCTAAG-5'
C
5'-CCAATG-3'
3'-GAATCC-5'
D
5'-CATTAG-3'
3'-GATAAC-5'
4
AIPMT 2012 Mains
MCQ (Single Correct Answer)
+4
-1
Change Language
Biolistics (gene-gun) is suitable for -
A
DNA finger printing
B
Constructing recombinant DNA by joining with vectors
C
Disarming pathogen vectors
D
Transformation of plant cells
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