1
IAT (IISER) 2023
MCQ (Single Correct Answer)
+4
-1
A 1000 base-pair DNA fragment was cloned between Hind III and EcoRI sites of the plasmid vector (plAT) of size 3500 base-pair. The cloned fragment had a Not I site as shown in the figure. In order to confirm the presence of the insert, the recombinant plasmid was digested completely with (a) Not I and EcoR I, and (b) Not I and Hind III.
In lane 1 the products of the digestion by Not I and EcoRI was loaded. In lane 2 the products of the digestion by Not I and Hind III was loaded. Which one of the following correctly represents the agarose gel electrophoresis profile of the digested recombinant plasmid for (a) and (b), respectively?
A
B
C
D
2
IAT (IISER) 2022
MCQ (Single Correct Answer)
+4
-1
As opposed to DNA replication within the cell, discontinuous synthesis of DNA does NOT occur in a polymerase chain reaction (PCR). Why?
Questions Asked from Biotechnology: Principles and Processes (MCQ (Single Correct Answer))
Number in Brackets after Paper Indicates No. of Questions
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