Biotechnology: Principles and Processes · Biology · IAT (IISER)

Which of the following plasmid vectors can be used for cloning of a gene, with restriction enzymes BamHI and EcoRI, and ampicillin-containing nutrient agar for selection? [Ori - origin of replication; $\mathrm{Amp}^R$ - gene for ampicillin resistance]

Polymerase chain reaction (PCR) is used to amplify a gene of interest (GOI). If, after 30 cycles of PCR, 1 billion copies of GOI are produced, approximately how many copies of GOI were present at the end of the $20^{\text {th }}$ cycle?