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IAT (IISER) 2022
MCQ (Single Correct Answer)
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-1
As opposed to DNA replication within the cell, discontinuous synthesis of DNA does NOT occur in a polymerase chain reaction (PCR). Why?
A
The replication fork is formed and DNA ligase activity of the Taq polymerase joins the discontinuous fragments.
B
The replication fork is formed and Taq polymerase extends DNA in both $3^{\prime}$ to $5^{\prime}$ and $5^{\prime}$ to $3^{\prime}$ direction.
C
Denaturation step in PCR substitutes for the replication fork and Taq polymerase extends DNA only in the $3^{\prime}$ to $5^{\prime}$ direction.
D
Denaturation step in PCR substitutes for the replication fork and Taq polymerase extends DNA only in the $5^{\prime}$ to $3^{\prime}$ direction.
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