1
IAT (IISER) 2024
MCQ (Single Correct Answer)
+4
-1

Which of the following plasmid vectors can be used for cloning of a gene, with restriction enzymes BamHI and EcoRI, and ampicillin-containing nutrient agar for selection? [Ori - origin of replication; $\mathrm{Amp}^R$ - gene for ampicillin resistance]

A
IAT (IISER) 2024 Biology - Biotechnology: Principles and Processes Question 1 English Option 1
B
IAT (IISER) 2024 Biology - Biotechnology: Principles and Processes Question 1 English Option 2
C
IAT (IISER) 2024 Biology - Biotechnology: Principles and Processes Question 1 English Option 3
D
IAT (IISER) 2024 Biology - Biotechnology: Principles and Processes Question 1 English Option 4
2
IAT (IISER) 2024
MCQ (Single Correct Answer)
+4
-1

Polymerase chain reaction (PCR) is used to amplify a gene of interest (GOI). If, after 30 cycles of PCR, 1 billion copies of GOI are produced, approximately how many copies of GOI were present at the end of the $20^{\text {th }}$ cycle?

A
10 million
B
0.66 billion
C
1 million
D
0.1 billion
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