1
IAT (IISER) 2023
MCQ (Single Correct Answer)
+4
-1
A 1000 base-pair DNA fragment was cloned between Hind III and EcoRI sites of the plasmid vector (plAT) of size 3500 base-pair. The cloned fragment had a Not I site as shown in the figure. In order to confirm the presence of the insert, the recombinant plasmid was digested completely with (a) Not I and EcoR I, and (b) Not I and Hind III.
In lane 1 the products of the digestion by Not I and EcoRI was loaded. In lane 2 the products of the digestion by Not I and Hind III was loaded. Which one of the following correctly represents the agarose gel electrophoresis profile of the digested recombinant plasmid for (a) and (b), respectively?
2
IAT (IISER) 2023
MCQ (Single Correct Answer)
+4
-1
The following pedigree chart shows the inheritance of a genetic disorder. I-2 and III-2 are the only affected individuals.
Which one of the following is the correct pattern of inheritance of the disorder, and the genotype of the II- 3 individual?

3
IAT (IISER) 2023
MCQ (Single Correct Answer)
+4
-1
$$ \text { How many radial nodes does } \mathrm{Ca}^{+} \text {have in its } 4 \text { s orbital? } $$
4
IAT (IISER) 2023
MCQ (Single Correct Answer)
+4
-1
Amongst $\mathrm{O}_2, \mathrm{~N}_2, \mathrm{~F}_2$, and $\mathrm{B}_2$, which molecules will be attracted to an external magnetic field?
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Biology
15
Chemistry
15
Mathematics
15
Physics
15
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