For which one of the following diseases does the causative agent require the splicing of their hnRNA to generate mature mRNA?

$$ \text { The diagram represents an enzyme, its substrate and potential inhibitors }(P, Q, R, S, T) \text {. } $$

Which one of the following combinations is the best pair of competitive inhibitors for the enzyme?

In an island with 10,000 individuals, four have sickle cell anemia, a recessive autosomal disease. Assuming that the locus is in Hardy-Weinberg equilibrium, how many individuals in that island are expected to be heterozygous for the disease allele?

A 1000 base-pair DNA fragment was cloned between Hind III and EcoRI sites of the plasmid vector (plAT) of size 3500 base-pair. The cloned fragment had a Not I site as shown in the figure. In order to confirm the presence of the insert, the recombinant plasmid was digested completely with (a) Not I and EcoR I, and (b) Not I and Hind III.

In lane 1 the products of the digestion by Not I and EcoRI was loaded. In lane 2 the products of the digestion by Not I and Hind III was loaded. Which one of the following correctly represents the agarose gel electrophoresis profile of the digested recombinant plasmid for (a) and (b), respectively?