$$ \text { The diagram represents an enzyme, its substrate and potential inhibitors }(P, Q, R, S, T) \text {. } $$

Which one of the following combinations is the best pair of competitive inhibitors for the enzyme?

In an island with 10,000 individuals, four have sickle cell anemia, a recessive autosomal disease. Assuming that the locus is in Hardy-Weinberg equilibrium, how many individuals in that island are expected to be heterozygous for the disease allele?

A 1000 base-pair DNA fragment was cloned between Hind III and EcoRI sites of the plasmid vector (plAT) of size 3500 base-pair. The cloned fragment had a Not I site as shown in the figure. In order to confirm the presence of the insert, the recombinant plasmid was digested completely with (a) Not I and EcoR I, and (b) Not I and Hind III.

In lane 1 the products of the digestion by Not I and EcoRI was loaded. In lane 2 the products of the digestion by Not I and Hind III was loaded. Which one of the following correctly represents the agarose gel electrophoresis profile of the digested recombinant plasmid for (a) and (b), respectively?

The following pedigree chart shows the inheritance of a genetic disorder. I-2 and III-2 are the only affected individuals. Which one of the following is the correct pattern of inheritance of the disorder, and the genotype of the II- 3 individual?