1
MHT CET (PCB) 2025 9th April Evening Shift
MCQ (Single Correct Answer)
+1
-0

In tissue culture technique, to maintain aseptic conditions the nutrient medium is autoclaved at constant pressure of _________ $\mathrm{lb} / \mathrm{sq}$ inch for continuous __________ minutes.

A

10,15

B

15,15

C

15,20

D

20,20

2
MHT CET (PCB) 2025 9th April Evening Shift
MCQ (Single Correct Answer)
+1
-0

$$ \text { Match the restriction enzyme in Column I with their recognition sequences in Column II. } $$

MHT CET (PCB) 2025 9th April Evening Shift Biology - Biotechnology: Principles and Processes Question 1 English Choose the correct option:
A

i-a, ii -d, iii -c, iv - b.

B

i - d, ii - b, iii - a, iv -c.

C

i-a, ii -c, iii -b, iv -d.

D

i-d, ii -a, iii -b, iv -c.

3
MHT CET (PCB) 2025 9th April Evening Shift
MCQ (Single Correct Answer)
+1
-0

The transfer of rDNA into a bacterial cell for the purpose of its cloning, is assisted by ________ ions.

A

$\mathrm{Mg}^{++}$

B

$\mathrm{Ca}^{++}$

C

$\mathrm{Cl}^{-}$

D

$\mathrm{K}^{+}$

4
MHT CET (PCB) 2025 9th April Morning Shift
MCQ (Single Correct Answer)
+1
-0

What is the time duration required for completion of one PCR Cycle?

A

1 to 2 minutes

B

3 to 4 minutes

C

5 to 6 minutes

D

7 to 8 minutes

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